Repeated fed-batch culture methods
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United States of America Patent
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Feb 16, 2021
Grant Date -
Apr 21, 2016
app pub date -
Oct 14, 2015
filing date -
Oct 16, 2014
priority date (Note) -
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Abstract
Provided herein are methods of culturing a microorganism. The method includes providing a container comprising one or more microorganisms and medium, wherein the microorganisms and medium form a start volume. The microorganisms and medium are cultured until the culture reaches a threshold indicator, wherein culturing comprises feeding one or more carbon sources to the culture and wherein the culture is at a threshold volume when the threshold indicator is reached. The method also includes harvesting a portion of the threshold volume to leave a residual volume that is 40% or less of the start volume and adding fresh medium to the container in an amount to return the volume of the culture to the start volume.
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