Semi-continuous culture methods
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United States of America Patent
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Jun 9, 2020
Grant Date -
Apr 21, 2016
app pub date -
Oct 14, 2015
filing date -
Oct 16, 2014
priority date (Note) -
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Abstract
Provided herein are methods of culturing a microorganism. The methods include providing a container comprising one or more microorganisms in a medium, which has a first carbon to nitrogen ratio; culturing the microorganisms until the culture reaches a threshold indicator; harvesting a portion of the culture while maintaining the majority of the culture in the container; and adding fresh medium comprising a second carbon to nitrogen ratio to the container with the majority of the culture comprising the microorganisms.
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