EXTRACELLULAR DITERPENE PRODUCTION

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United States of America Patent

SERIAL NO

14893284

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Abstract

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The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism in a suitable fermentation medium, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol, whereby a diterpene or glycosylated diterpene is produced extracellularly in the fermentation medium; and b. recovering the diterpene or glycosylated diterpene from the fermentation medium.

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Patent Owner(s)

Patent OwnerAddress
DSM IP ASSETS B VHOLLAND HEERLEN HEERLEN LIMBURG

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
BOER, Viktor Marius Echt, NL 32 168
BROERS, Nicolette Jasmijn Echt, NL 8 32
LAWRENCE, Adam G Echt, NL 9 44

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