METHOD FOR MEASURING RELATIVE CONTENT OF GRAM-NEGATIVE BACTERIA IN ACTIVE SLUDGE
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United States of America Patent
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Mar 31, 2016
app pub date -
Sep 13, 2015
filing date -
Sep 30, 2014
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Abstract
A method for measuring a relative content of Gram-negative bacteria in an active sludge, the method including: 1) breaking zoogloeas in the active sludge; 2) isolating 2-keto-3-deoxyoctonic acid (KDO) from free bacteria and cell debris; 3) performing fluorescence labeling on the isolated KDO to obtain a fluorescent derivative; and 4) isolating the fluorescent derivative by high performance liquid chromatography (HPLC) and conducting quantitative measurement by fluorescence to obtain the relative content of the Gram-negative bacteria in the active sludge.
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