CONCENTRATION DEVICE FOR MICROORGANISMS IN LARGE VOLUMES OF TURBID WATER AND METHOD THEREFOR
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United States of America Patent
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Issued Date -
Mar 31, 2016
app pub date -
Sep 25, 2014
filing date -
Sep 25, 2014
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Abstract
A device and method for concentrating microorganisms in large volumes of turbid water. When particulate builds up at the inlet filter header and pressure increases, filtrate that has collected within the filtrate reservoir serves as backflush fluid. The hollow fiber filter cartridge is backflushed as the pump reverses and filtrate flows from the filtrate reservoir, back through the hollow fiber filter cartridge, and out the inlet filter header, thereby dislodging the accumulated particulate. Elution solution is pumped though the device to remove microorganisms that may have adhered to the filter fibers and tubing. The retentate is then recovered by forward flushing the filter with eluting solution so that the retentate is sent to the off-line retentate sample container. There, the retentate sample is isolated and prevented from re-entering the hollow fiber filter cartridge. The inlet filter header therefore serves as an in-line pre-filter.
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