Sequence specific primer pool for multiplex PCR and method of detecting microbial infections in thalassemia patients
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United States of America Patent
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Jan 9, 2018
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Oct 20, 2014
filing date -
Sep 19, 2014
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Abstract
Reagents and methods for the fast, accurate and early detection of infections in thalassemia major patients. Reagents include a primer pool containing a mixture of primer pairs for the specific recognition and simultaneous amplification of targeted gene sequences of pathogens from a biological sample collected from a patient in a multiplex PCR reaction. Pathogens may include at least one Fungi species, Klebsiella pneumoniae, Candida albicans and Staphylococcus aureus. Subsequent identification of the pathogens is achieved by DNA sequencing analysis.
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