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Abstract

Disclosed are methods and materials for detecting RNA in a sample. In some forms, the method involves (a) bringing into contact the sample and a probe array, (b) bringing into contact the probe array and a ribonuclease specific for RNA/DNA hybrids (such as RNase H), (c) bringing into contact the probe array, labeled nucleotides, and a nucleic acid polymerase capable of extending a RNA strand using a DNA template and capable of incorporating the labeled nucleotides in the extension from the RNA strand (such as Klenow fragment DNA polymerase), and (d) detecting the labeled nucleotides in the extended nucleic acid strand. The probe array comprises one or more chimeric probes. The chimeric probes comprise a DNA region and a RNA region, where the DNA region and the RNA region are contiguous and where the DNA region is 5′ of the RNA region. The chimeric probe can also include a second DNA region. The second DNA region can also be contiguous with the RNA region and can be 3′ of the RNA region.

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First Claim