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Abstract

The present invention is concerned a method for cultivation of Chlorella pyrenoidosa or organisms derived from Chlorella pyrenoidosa, comprising steps of a) providing a fermenter containing primarily a food waste hydrolysate medium, b) at the beginning of a first phase, introducing a strain of Chlorella pyrenoidosa or Chlorella pyrenoidosa derived organisms in the food waste hydrolysate medium for cultivation, wherein the food waste hydrolysate medium in the first phase contains substantially 5-30 g L⁻¹ glucose, 74-144 mg L⁻¹ free amino nitrogen and 40-64 mg L⁻¹ phosphate, c) during the first phase, allowing diminishing of free amino nitrogen and/or phosphate in the food waste hydrolysate medium until the free amino nitrogen and/or phosphate becomes limited, and d) at the beginning of a second phase after the first phase, feeding a fresh supply of food waste hydrolysate medium in the fermenter, wherein the fresh supply of food waste hydrolysate contains substantially 30-90 g L⁻¹ glucose, 160-230 mg L⁻¹ free amino nitrogen and 80-270 mg L⁻¹ phosphate, and feeding occurs at a certain dilution rate.

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First Claim