SCREENING METHOD FOR ION CHANNEL MODULATORS USING MUTATED BKCA CHANNEL
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
N/A
Issued Date -
Mar 3, 2016
app pub date -
Dec 10, 2013
filing date -
Apr 10, 2013
priority date (Note) -
Published
status (Latency Note)
Importance
Abstract
The present disclosure relates to a doubly mutated BKCa channel construct and a novel cell-based screening system for ion channel modulators using the same. A doubly mutated BKCa channel-comprising cell-based system of the present invention, when compared to a control group, shows remarkably increased fluorescence caused by membrane depolarization, regardless of whether there is a separate increase of [Ca2+]i, and shows activity (movement in the negative direction of a G/V curve) that is further triggered by a known activator (for example, CTBIC). Therefore, the system of the present disclosure is capable of more effectively and accurately analyzing the activity of an ion channel, and thus can be effectively applied not only to the separation/identification of ion channel modulators but also to screening for a therapeutic agent for a condition, disease or disorder related to the modulation of a BKCa channel.
First Claim
Family
International Classification(s)
Cited Art Landscape
Patent Citation Ranking
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully