Method for Differentiation of Polynucleotide Strands
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United States of America Patent
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Issued Date -
Feb 25, 2016
app pub date -
Aug 31, 2015
filing date -
Jul 6, 2009
priority date (Note) -
Published
status (Latency Note)
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Abstract
Objective of the present invention is to provide a method for keeping of directional information in double-stranded DNA. We suggest to convert polynucleotide into a hybrid double-stranded DNA. One particular strand of this hybrid double-stranded DNA should be synthesised using at least one modified nucleotide. Thus, this particular strand would contain modified nucleotides along the whole length. Density of directional markers would not depend on the length of polynucleotides. Any internal fragments of the hybrid double-stranded DNA would have directional information. When it is necessary the modified strand may be easily degraded or separated from the other strand. It was found that such hybrid double-stranded DNA may be easily generated in a number of molecular biology tasks and may be used for molecular cloning, library preparation and strand separation.
First Claim
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Family
| Country | kind | publication No. | Filing Date |
|---|---|---|---|
| EP | A1 | EP2272976 | Jul 06, 2009 |
| US | B2 | US9175336 | Jul 06, 2010 |
| WO | A1 | WO2011003630 | Jul 06, 2010 |
| EP | B1 | EP2451973 | Jul 06, 2010 |
| US | B1 | US8999677 | Nov 18, 2014 |
International Classification(s)
- 2015 Application Filing Year
- C12Q Class
- 3483 Applications Filed
- 2244 Patents Issued To-Date
- 64.43 % Issued To-Date
Cited Art Landscape
Patent Citation Ranking
- 1 Citation Count
- C12Q Class
- 46.01 % this patent is cited more than
- 9 Age