METHODS FOR IDENTIFYING PROTEINS AND COMPOUNDS THAT MODULATE THE ACTIVITY OF OTUB1

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United States of America Patent

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14785429

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Abstract

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The present invention describes that OTUB1 cleavage of K48 poly-ubiquitin is stimulated by a select subset of E2 enzymes, and that this stimulation is regulated by the ubiquitin-charged state of the E2 and free ubiquitin. Structural and biochemical studies of OTUB1 and UBCH5B show that the E2 stimulates binding of the polyubiquitin substrate by contacting the OTUB1 N-terminal ubiquitin-binding helix. Methods for identifying E2 enzymes which stimulate or inhibit cleavage of K48 polyubiquitin, as well as novel target compounds which modulate this interaction are provided.

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Patent Owner(s)

Patent OwnerAddress
THE JOHNS HOPKINS UNIVERSITY3400 N CHARLES STREET BALTIMORE MARYLAND 21218 UNITED STATES OF AMERICA

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  • 2014 Application Filing Year
  • C12Q Class
  • 3114 Applications Filed
  • 2141 Patents Issued To-Date
  • 68.76 % Issued To-Date
Click to zoom InYear of Issuance% of Matters IssuedCumulative IssuancesYearly Issuances201420152016201720182019202020212022202320240255075100

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Dibello, Anthony Baltimore, US 2 6
Wiener, Reuven Herzeliya, IL 1 0
Wolberger, Cynthia Baltimore, US 3 2

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Citation count rangeNumber of patents cited in rangeNumber of patents cited in various citation count ranges2395234329687311986733701 - 1011 - 2021 - 3031 - 4041 - 5051 - 6061 - 7071 - 8081 - 9091 - 100100 +0200400600800100012001400160018002000220024002600

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