LIGHT SHEET FLUORESCENCE AND DIFFERENTIAL INTERFERENCE CONTRAST MICROSCOPE
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United States of America Patent
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Aug 5, 2015
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Aug 6, 2014
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One or more excitation optical beams are scanned or otherwise directed to a specimen volume to establish a light sheet. Fluorescence from a specimen portion in the light sheet is collected and used to form an image. An interference contrast image of the same or an adjacent specimen portion is obtained by directing an interference contrast optical beam to the specimen so as to be substantially perpendicular to the light sheet. A common imaging device can be used to capture both images, and the resulting image permits correlation of fluorescence image features with specimen structure.
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