Assay Enhancement by Selective Deposition and Binding on Amplification Structures
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United States of America Patent
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Feb 4, 2016
app pub date -
Mar 15, 2014
filing date -
Mar 15, 2013
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This disclosure provides, among other things, a method for enhancing detection of an analyte that is bound to a substrate comprising a signal amplification layer on a surface of the substrate, wherein the signal amplification layer comprises high-amplification regions and low-amplification regions, and the high-amplification regions amplify signals at said surface more than the low-amplification regions. The method comprises selectively masking the low-amplification regions of the substrate, thereby increasing the probability that an analyte will bind to a high-amplification region and be detected.
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