T7 RNA POLYMERASE VARIANTS WITH ENHANCED THERMOSTABILITY
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Oct 16, 2015
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Apr 16, 2010
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Abstract
The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous.
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