FRAGMENT COMPLEMENTATION OF BASED ASSAYS
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United States of America Patent
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Issued Date -
Jan 28, 2016
app pub date -
Mar 13, 2014
filing date -
Mar 13, 2013
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Abstract
The present disclosure provides, among other things, methods and compositions for detecting and/or quantifying analytes using fragment complementation technologies. In accordance with various embodiments of the present disclosure, a kit can include a) a capture probe immobilized on a surface, wherein the capture probe can associate with an analyte in a sample, thereby forming at least one captured analyte; b) a detection element including a target interacting probe associated with a first subunit of a detectable entity, wherein the target interacting probe can associate with the captured analyte so that a first complex is formed; and c) a second subunit that can complement the first subunit and generate a detectable entity, wherein the presence and/or amount of the analyte is indicated by detecting level or activity of the detectable entity.
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