METHOD FOR FABRICATING A CELL-LADEN HYDROGEL CONSTRUCT
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United States of America Patent
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Issued Date -
Jan 28, 2016
app pub date -
Jul 22, 2014
filing date -
Jul 22, 2014
priority date (Note) -
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Abstract
The present invention provides a method for fabricating a cell-laden hydrogel construct, comprising the steps of: (a) making a mixture of a physically gelable protein, a first crosslinking enzyme and a cell; (b) extruding the mixture into a second crosslinking enzyme before the mixture is gelled and forming a microgel by physical gelling; and (c) reacting the microgel with the second crosslinking enzyme to fabricate the cell-laden hydrogel construct.
First Claim
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