SEX SORTED SPERM DEMONSTRATING A DOSE RESPONSE AND METHODS OF PRODUCING SEX SORTED SPERM DEMONSTRATING A DOSE RESPONSE
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United States of America Patent
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Issued Date -
Jan 14, 2016
app pub date -
Sep 22, 2015
filing date -
Mar 4, 2013
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Abstract
Embodiments of the claimed invention relate to a method of producing sex sorted sperm having an improved dose response which includes the steps of extending a sperm sample with a buffering holding media and adjusting the concentration of the extended sperm sample to a target concentration range. The extended sperm may then be stained with a DNA selective dye and sex sorted with a flow cytometer into a catch fluid. During the extending and sorting of the sperm sample, the pH may be maintained at a target pH. Additionally, at least one of the buffering holding media, the DNA selective dye and the catch fluid may be supplemented with at least one antioxidant.
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