Method for controlling length of overhang of double stranded DNA
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United States of America Patent
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Jan 16, 2018
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app pub date -
Oct 28, 2013
filing date -
Feb 8, 2013
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Abstract
Disclosed are methods and kits for controlling the length of overhangs at the ends of double stranded DNA based on a competitive inhibition. Further provided are ligase-independent methods for joining the two ends of DNA strands using the same. The present methods efficiently control the overhangs of ds DNA. This user-controlled overhangs supply a tool for efficient ligation in a ligase independent way and can be advantageously used in DNA recombinant technology such as cloning gene or DNA fragments of interest or PCR products.
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