DNA TEMPLATES FOR SMALL RNA PRODUCTION IN MAMMALIAN CELLS
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Aug 21, 2015
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Oct 12, 2010
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Abstract
This disclosure describes unique single stranded DNA templates having a characteristic sequence and secondary structure. The DNA templates disclosed herein are useful for making small RNA molecules through promoterless transcription by a mammalian RNA polymerase, and can serve as an effective vector for producing small RNA molecules of interest in vitro, in situ and in vivo in mammalian cells.
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