SUPERCOILED MINIVECTORS AS A TOOL FOR DNA REPAIR, ALTERATION AND REPLACEMENT
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United States of America Patent
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Issued Date -
Dec 31, 2015
app pub date -
May 30, 2013
filing date -
May 30, 2012
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Abandoned
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Abstract
In some embodiments the present disclosure provides a composition for targeted alteration of a DNA sequence and methods of altering the targeted DNA sequence using the composition. In some embodiments such a composition comprises a MiniVector comprising a nucleic acid sequence template for homology-directed repair, alteration, or replacement of the targeted DNA sequence within a cell in vivo or in vitro, where the MiniVector lacks both a bacterial origin of replication and an antibiotic selection gene, and wherein the Mini Vector has a size up to about 2,500 base pairs.
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