TEVI CHIMERIC ENDONUCLEASE AND THEIR PREFERENTIAL CLEAVAGE SITES
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Jan 29, 2014
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Feb 1, 2013
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Abstract
The present invention relates to a method to cleave target nucleic acid sequence by the catalytic domain of a GIY-YIG homing endonucleases 1-Tevl. More precisely, the invention relates to the deciphering of new preferential 1-Tevl cleavage sites for efficient and specific cleavage activity. The invention concerns a method for the generation of TevI specific chimeric endonucleases to target nucleic acid sequence including such cleavage sites and methods of using same for gene editing.
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