METHODS OF USING ION EXCHANGE CHROMATOGRAPHY TO CONTROL LEVELS OF HIGH MANNOSE GLYCOFORMS
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United States of America Patent
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Dec 17, 2015
app pub date -
Dec 18, 2013
filing date -
Dec 20, 2012
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Provided are methods of using ion exchange based chromatography to control the levels of high mannose glycoforms in a glycoprotein sample, such as an antibody sample. The high mannose glycoforms can be removed from the sample or isolated and enriched. Also provided is a composition comprising a glycoprotein, such as an antibody, from which the high mannose glycoforms have been reduced or removed or isolated or enriched using an ion exchange based chromatography step.
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