Method for Nondestructive Detection of MiRNA Expression in Cell and Determination of Cell Type and State
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United States of America Patent
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Dec 10, 2015
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May 20, 2013
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Jul 24, 2012
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Abstract
The present invention provides a method for non-invasively detecting the expression levels of miRNAs in cell media and determining the types and status of the cells, and specifically provides a method for determining the type and status of the cells according to the expression level of miRNAs in a cell medium. The method comprises: culturing different types of cells, collecting the medium of the cells, extracting RNAs from the medium, performing reverse transcription on the RNAs, detecting miRNAs in the cell medium by the fluorescent quantitative PCR method, and determining the types and status of the detected cells according to the relationship between the expression levels of miRNAs in different types and status of cells and the expression level of the detected miRNAs. The method of the present invention proves for the first time that the expression levels of miRNAs in the medium can be detected to determine the types and status of cells, comprising the pluripotency level of stem cells and the status of cells obtained through transdifferentiation, so as to avoid causing damages to cells, and is especially suitable for the experimental and clinical applications in which the number of cells is limited.
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