PORTABLE FLUORESCENCE DETECTION SYSTEM AND MICROASSAY CARTRIDGE
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United States of America Patent
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Issued Date -
Dec 3, 2015
app pub date -
Dec 20, 2013
filing date -
Dec 21, 2012
priority date (Note) -
Published
status (Latency Note)
Importance
Abstract
Disclosed is a compact, microprocessor-controlled instrument for fluorometric assays in liquid samples, the instrument having a floating stage with docking bay for receiving a microfluidic cartridge and a scanning detector head with on-board embedded microprocessor operated under control of a ODAP daemon resident in the detector head for controlling source LEDs, emission signal amplification and filtering in an isolated, low noise, high-gain environment within the detector head. Multiple optical channels may be incorporated in the scanning head. In a preferred configuration, the assay is validated using dual channel optics for monitoring a first fluorophore associated with a target analyte and a second fluorophore associated with a control. Applications include molecular biological assays based on PCR amplification of target nucleic acids and fluorometric assays in general, many of which require temperature control during detection.
First Claim
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