PARTIAL PURIFICATION METHOD FOR HYDROPHILIC RECOMBINANT PROTEIN
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United States of America Patent
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Dec 3, 2015
app pub date -
Dec 18, 2013
filing date -
Dec 27, 2012
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Abstract
The present invention relates to a method for partially purifying a hydrophilic recombinant protein from a host expressing a hydrophilic recombinant protein. The method includes: a solution acquisition step in which a dissolving solvent is added to the host expressing a hydrophilic recombinant protein so as to dissolve the host cell and an insoluble substance is separated therefrom so as to obtain a solution. The hydrophilic recombinant protein has a hydropathy index of 0 or less, and the dissolving solvent is an aprotic polar solvent. Preferably, the solution is subjected to solvent substitution using an aqueous solvent.
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