METHOD FOR PRODUCING INDUCED PLURIPOTENT STEM CELLS, CARDIOMYOCYTES OR PRECURSOR CELLS THEREOF
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United States of America Patent
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Issued Date -
Nov 26, 2015
app pub date -
Dec 27, 2013
filing date -
Dec 28, 2012
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Abstract
Nuclear reprogramming substances are contacted with a somatic cell and, after culture, cell population is fractionated based on the expression of Sca-1 or EpCam and CD34. By sorting Sca-1-negative CD34-negative cells or EpCam-positive CD34-negative cells, a cell population having high possibility of iPS cell formation can be selected. On the other hand, Sca-1-positive CD34-positive cells or EpCam-negative CD34-positive cells are useful as a source of myocardial cell or a progenitor cell thereof having a low risk of tumor formation.
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