METHOD OF DIAGNOSING BACTERIAL INFECTIONS USING BACTERIAL GLYCOPROTEINS
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United States of America Patent
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Aug 13, 2012
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Aug 12, 2011
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Abstract
The present application provides a method of diagnosing bacterial infections using engineered glycoproteins in an immunoassay. The engineered bacterial glycoproteins used in the immunoassay comprise a bacterial antigen covalently attached to a protein via polysaccharyltransferase (PTase)-mediated glycosylation, wherein the bacterial antigen is selected based on the bacterial infection of interest. Antibodies in a bodily fluid of subjects infected with a bacteria will bind to the engineered glycoprotein, and the resulting binding complexes are detected or quantitated.
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