Synthetic Nucleic Acids for Polymerization Reactions
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United States of America Patent
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Nov 5, 2015
app pub date -
Jul 13, 2015
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Sep 1, 2011
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Compositions and methods are provided for inhibiting a DNA binding enzyme from reacting with non-target DNA at a temperature below the reaction temperature. The inhibitor is a synthetic nucleic acid which is single stranded but may fold to form at least one double stranded region designed to melt at a temperature which is lower than the reaction temperature, and at least one single stranded region where the single stranded region at the 5′ end contains at least one unnatural and/or modified nucleotide and optionally a sequence at the 3′ end contains one or more derivative nucleotide or linkages.
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