Refolding Proteins Using a Chemically Controlled Redox State
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United States of America Patent
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Jan 30, 2015
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Jun 22, 2009
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Abstract
A method of refolding proteins expressed in non-mammalian cells present in concentrations of 2.0 g/L or higher is disclosed. The method comprises identifying foe thiol pair ratio and the redox buffer strength to achieve conditions under which efficient folding at concentrations of 2.0 g/L or higher is achieved and can be employed over a range of volumes, including commercial scale.
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