DETECTION METHOD FOR HYDROXYMETHYLATED CYTOSINE IN DNA AND REAGENT KIT FOR DETECTION
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United States of America Patent
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Oct 29, 2015
app pub date -
Dec 24, 2013
filing date -
Dec 28, 2012
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Abstract
The present invention is to provide a method for detecting a hydroxymethylated cytosine in DNA and a detection kit therefor. The present invention relates to “a method for detecting the hydroxymethylated cytosine in DNA, which the method comprises: (1) a step in which a single-stranded DNA is contacted with (i) a polyvalent metal oxide or a polyvalent metal acid salt of a metal atom selected from group 6, group 8, group 9 and group 10 of the periodic table, and contacted with (ii) a peroxide selected from persulfuric acid, percarboxylic acids and the salts thereof; (2) a step in which a specific region of the single-stranded DNA treated in (1) is subjected to amplification treatment; (3) a step in which the presence or absence of an objective amplification product obtained in (2) is detected; and (4) a step in which on the basis of the results of (3), the presence or absence of hydroxymethylated cytosine in the specific region of DNA is determined.”, “a reagent kit for detecting hydroxymethylated cytosine in DNA comprising of a reagent including the above polyvalent metal oxide or a polyvalent metal acid salt and the above peroxide”.
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