METHOD FOR MEASURING ORGANISM-ORIGINATED PHYSIOLOGICALLY ACTIVE SUBSTANCE IN HYALURONIC ACID PREPARATION
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United States of America Patent
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Issued Date -
Oct 22, 2015
app pub date -
May 7, 2013
filing date -
May 9, 2012
priority date (Note) -
Abandoned
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Abstract
Provided is a technique for achieving a higher measurement accuracy in the detection of an organism-originated physiologically active substance or the measurement of the concentration of the organism-originated physiologically active substance in a hyaluronic acid preparation. The present invention relates to a measurement method for detecting an organism-originated physiologically active substance or measuring the concentration of the organism-originated physiologically active substance in a hyaluronic acid preparation by mixing the hyaluronic acid preparation with an AL reagent to allow the organism-originated physiologically active substance in the hyaluronic acid preparation and an AL to react with each other. A mixed solution of the hyaluronic acid preparation with the AL reagent is diluted with an aqueous electrolyte solution, and a specific protein having a higher affinity for the organism-originated physiologically active substance than that of hyaluronic acid is added to the mixed solution.
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