Quantitative Reverse Transcription Polymerase Chain Reaction Kit for Breast Cancer Drug Screening Test and Early Diagnosis Using Tissue and Blood
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United States of America Patent
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Issued Date -
Oct 22, 2015
app pub date -
Sep 25, 2013
filing date -
Sep 24, 2013
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Abandoned
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Abstract
A method for a breast cancer drug screening including separating a full-length RNA from a cell obtained from a tissue or blood of a suspected cancer patient; synthesizing a cDNA from the full-length RNA; performing a PCR with the synthesized cDNA by using a composition including sets of a Pair of primers and a probe, each set independently able to amplify human epidermal growth factor receptor 2 (HER2), cytokeratin 19, EpCAM, a hTERT, Ki67, vimentin, and a GAPDH. The method further includes comparing an amount of the amplification with an expression amount to a normal person. A kit for performing methodology of the invention.
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