RNA DETECTION METHOD AND DETECTION KIT
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United States of America Patent
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Issued Date -
Oct 22, 2015
app pub date -
May 13, 2013
filing date -
May 15, 2012
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Abandoned
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Abstract
Provided are a method and kit for detecting RNA, which allow RNA such as microRNA and messenger RNA to be detected with high sensitivity and simply, and signal amplification probes to be used in the method. Specifically, provided is a method of detecting target RNA, the method including: (A) a capturing step of capturing RNA by a capture substance for capturing the RNA; (B) a complex-for-detection forming step of subjecting the captured RNA and signal amplification probes capable of self-assembling to a reaction to form a complex for detection including the RNA, the capture substance, and a probe polymer formed from the signal amplification probes; and (C) a detecting step of detecting the probe polymer bound in the complex for detection to detect the RNA, one of the signal amplification probes including a poly(T) sequence.
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