HIGHLY EFFICIENT PRODUCTION OF LACTIC ACID FROM HEMICELLULOSE SUGARS BY NEWLY ISOLATED THERMOPHILIC BACILLUS COAGULANS STRAINS
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United States of America Patent
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Oct 22, 2015
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Nov 22, 2013
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Nov 23, 2012
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Abstract
The present invention relates to newly isolated Bacillus coagulans strains C106, JI 12 and WCPlO-4, cost effective methods for producing lactic acid using the strains as well as seed inoculums and kits comprising the strains. Optically pure L-lactic acid was produced from xylose or glucose at high product titers (>200 g/L) and productivities (4.2 g/L/h on xylose and 4.6 g/L/h on glucose) at 50° C. When an acid hydrolysate (85 g sugars/L) of empty fruit bunch of oil palm trees was used, the L-lactic acid titer, yield and productivity reached 84.5 g/L, 97% and 1.84 g/L/h, respectively. A one-step, one-pot open fermentation was also developed to produce optically pure L-lactic acid from starch. The final lactic acid titer and productivity were as high as 202.0 g/L and 5.4 g/L/h, respectively. No glucose remained in the broth and the lactic acid yield reached 98%.
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