NUCLEIC ACID MOLECULES FOR HIGHLY SENSITIVE DETECTION OF LIGANDS, SCREENING METHOD FOR NUCLEIC ACID MOLECULES, AND OPTIMIZATION METHOD FOR SENSITIVITY OF NUCLEIC ACID MOLECULES
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United States of America Patent
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Issued Date -
Oct 15, 2015
app pub date -
Apr 25, 2013
filing date -
Apr 27, 2012
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Abandoned
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Abstract
It is an object of the present invention to provide nucleic acid molecules that enable highly sensitive detection of ligands (e.g., patulin). It is another object of the present invention to provide a screening method for nucleic acid molecules that enable highly sensitive detection of ligands (e.g., patulin), and a method for screening for nucleic acid molecules used for the optimization of nucleic acid molecules that enable highly sensitive detection of ligands (e.g., patulin). It is a further object of the present invention to provide a method for effectively removing ligands from samples containing ligands (e.g., patulin). According to the present invention, there is provided a loop-structured nucleic acid molecule for detection of ligands (e.g., patulin) having a DNA aptamer and a DNAzyme, wherein the sequence is modified between the DNA aptamer region and the DNAzyme.
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