METHOD FOR DETERMINING KINASE ACTIVITY

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United States of America Patent

APP PUB NO 20150276740A1
SERIAL NO

14632517

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Abstract

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Disclosed is a method for determining kinase activity. The method comprises: (A) mixing a specimen that comprises a kinase, a substrate of the kinase and an adenosine triphosphate (ATP) derivative that comprises a dinitrophenyl (DNP) group to obtain a mixture that comprises a DNP group-containing substrate in which the DNP group is introduced in the substrate; (B) mixing the mixture obtained at the (A) and an antibody that binds to the DNP group to form a complex that comprises the DNP group-containing substrate and the antibody; and (C) determining an activity of the kinase by detecting the complex. The ATP derivative is a compound in which the DNP group is bound to a phosphate group at a gamma position of ATP via a linker.

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SYSMEX CORPORATIONKOBE-SHI HYOGO 651-0073

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  • 2015 Application Filing Year
  • G01N Class
  • 11310 Applications Filed
  • 8345 Patents Issued To-Date
  • 73.79 % Issued To-Date
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Inventor Name Address # of filed Patents Total Citations
HARADA, Shigeyoshi Kobe, JP 4 12

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