METHOD FOR DETERMINING KINASE ACTIVITY
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United States of America Patent
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Issued Date -
Oct 1, 2015
app pub date -
Feb 26, 2015
filing date -
Mar 31, 2014
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Abstract
Disclosed is a method for determining kinase activity. The method comprises: (A) mixing a specimen that comprises a kinase, a substrate of the kinase and an adenosine triphosphate (ATP) derivative that comprises a dinitrophenyl (DNP) group to obtain a mixture that comprises a DNP group-containing substrate in which the DNP group is introduced in the substrate; (B) mixing the mixture obtained at the (A) and an antibody that binds to the DNP group to form a complex that comprises the DNP group-containing substrate and the antibody; and (C) determining an activity of the kinase by detecting the complex. The ATP derivative is a compound in which the DNP group is bound to a phosphate group at a gamma position of ATP via a linker.
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