ACYL-COA DEHYDROGENASE ASSAYS
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United States of America Patent
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Issued Date -
Sep 24, 2015
app pub date -
Oct 30, 2013
filing date -
Nov 5, 2012
priority date (Note) -
Abandoned
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Abstract
A method for conducting medium-chain acyl-CoA dehydrogenase (MCAD) and very-long-chain acyl-CoA dehydrogenase (VCAD) enzymatic activity assays is provided. The method may include, but is not limited to, preparing a sample; preparing an enzyme-specific substrate/reagent mixture; mixing an aliquot of the prepared sample with an aliquot of the enzyme-specific substrate/reagent mixture; reading absorbance in the range of about 600 nm; incubating the prepared sample and enzyme-specific substrate/reagent mixture; and reading absorbance in the range of about 600 nm at various time intervals.
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