Genetically engineered yeast
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United States of America Patent
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Jan 16, 2018
Grant Date -
Sep 24, 2015
app pub date -
Oct 10, 2013
filing date -
Oct 11, 2012
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Abstract
A genetically modified Saccharomyces cerevisiae including an active fermentation pathway producing 3-HP expresses an exogenous gene expressing the aminotransferase YhxA from Bacillus cereus AH1272 catalyzing a transamination reaction between beta-alanine and pyruvate to produce malonate semialdehyde. The yeast may also express a 3-hydroxyisobutyrate dehydrogenase (HIBADH) and a 3-hydroxypropanoate dehydrogenase (3-HPDH) and aspartate 1-decarboxylase. Additionally the yeast may express pyruvate carboxylase and aspartate aminotransferase.
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