RAPID DETECTION AND QUANTITATION OF PATHOGEN-SPECIFIC BIOMARKERS USING NANOPOROUS DUAL- OR MULTI-LAYER SILICA FILMS
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United States of America Patent
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Issued Date -
Sep 17, 2015
app pub date -
May 22, 2015
filing date -
Nov 30, 2012
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Abstract
Improved methods for detecting active tuberculosis are disclosed. A method comprises enriching at least one M. tuberculosis-specific biomolecule from a sample by contacting the sample with a nanoporous film; and detecting the presence of the M. tuberculosis-specific biomolecule or fragment(s) thereof. The method may further comprise digesting the enriched M. tuberculosis-specific biomolecule with an enzyme to produce a digestion product comprising at least one fragment of the M. tuberculosis-specific biomolecule. Improved sensitivity and speed achieved.
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