COMPOSITIONS AND METHODS FOR LONG INSERT, PAIRED END LIBRARIES OF NUCLEIC ACIDS IN EMULSION DROPLETS
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United States of America Patent
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Issued Date -
Sep 17, 2015
app pub date -
Mar 20, 2015
filing date -
Sep 21, 2012
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Abandoned
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Abstract
The invention provides for methods for uniquely labeling populations of nucleic acids of interest in emulsion droplets using random combinations of oligonucleotides. The labeling methodology of the invention may be used, inter alia, to generate mate pair genomic fragments without the need for circularization. Because the method is independent of circularization, mate pairs can be generated from any length genomic fragment.
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