HORMONE RESPONSIVE TISSUE CULTURE SYSTEM AND USES THEREOF
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United States of America Patent
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Sep 17, 2015
app pub date -
Dec 15, 2014
filing date -
May 7, 2004
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The invention provides tissue culture system for primary cells (e.g. normal mammalian primary epithelial progenitors). This system includes: a) a serum-free, chemically defined cell culture media; and, b) methods for isolation and in vitro long-term propagation of primary cells (e.g. primary epithelial cells). Primary cells so isolated and cultured can be kept undifferentiated and proliferate for many weeks (>15 weeks) or population doubling (>35 PD) without senescence, or any detectable genetic alterations. Upon changing media/culture conditions, these cells can be induced to differentiate.
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