METHOD FOR SCREENING MICRORNAS WITH GENE SILENCING FUNCTION AT BOTH LEVELS OF TRANSCRIPTION AND POST-TRANSCRIPTION
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United States of America Patent
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Issued Date -
Sep 10, 2015
app pub date -
Jul 26, 2013
filing date -
Jul 26, 2012
priority date (Note) -
Abandoned
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Abstract
Provided is a method for screening microRNAs with gene silencing function at both transcriptional and post-transcriptional levels. The microRNA obtained via the screening method can down-regulate the expression of mRNA of a target gene by binding to a specific site of 5′-flanking region of the target gene, and also down-regulate the expression of the protein of the target gene by binding to a specific site of the 3′-untranslated region of the target gene. Also provided are microRNAs obtained via the screening method, the use of the same in gene silencing, and a method of gene silencing by using the same.
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