NUCLEIC ACID AMPLIFICATION METHOD
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United States of America Patent
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Sep 3, 2015
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Feb 27, 2015
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Feb 28, 2014
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Abstract
A nucleic acid amplification method includes: forming a first liquid droplet containing a first reaction reagent for amplifying a first nucleic acid in one vessel containing a silicone oil to which a fluoro-modified silicone resin is added; forming a second liquid droplet containing a second reaction reagent for amplifying a second nucleic acid in the one vessel; and performing a nucleic acid amplification reaction in the vessel by fitting the one vessel in a nucleic acid amplification reaction apparatus while maintaining the first liquid droplet independent of the second liquid droplet and then operating the nucleic acid amplification reaction apparatus.
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