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Abstract

The present invention discloses a DL-alanine-producing engineering bacterium. This DL-alanine-producing engineering bacterium itself was inactivated in lactate dehydrogenase, pyruvate formate lyase, alcohol dehydrogenase, acetate kinase, fumarate reductase, alanine racemase, and methylglyoxal synthase; moreover, onto the chromosome thereof an exogenous L-alanine dehydrogenase gene and alanine racemase gene were integrated. In the present application, by integrating the exogenous L-alanine dehydrogenase gene into the chromosome of the engineering bacterium, the intermediate of glycolysis, pyruvic acid, was converted into L-alanine; by further integrating the exogenous alanine racemase gene, partial L-alanine was converted into D-alanine, achieving the direct production of DL-alanine from raw material saccharides, thereby decreasing the production cycle of DL-alanine, and increasing the yield of DL-alanine.

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First Claim