Method of producing lipids using a thioesterase variant
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United States of America Patent
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May 10, 2016
Grant Date -
Sep 3, 2015
app pub date -
Aug 22, 2013
filing date -
Sep 20, 2012
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Abstract
A method of producing a lipid, comprising steps of
- introducing a gene that encodes the following protein (a) or (b) into a host, and thereby obtaining a transformant, andcollecting a lipid from the transformant: (a) A protein comprising an amino acid sequence of the 112nd to 414th amino acids set forth in SEQ ID NO: 1 in which an amino acid corresponding to the 209th amino acid set forth in SEQ ID NO: 1 is substituted from tryptophan to threonine, glutamic acid or alanine, and having thioesterase activity, and(b) A protein comprising an amino acid sequence of the protein (a) in which one to several amino acids other than the amino acid corresponding to the 209th amino acid set forth in SEQ ID NO: 1 are deleted, substituted, inserted or added, and having thioesterase activity.
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