Rapid mincing of adipose tissues to isolate live cells in vitro

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United States of America Patent

PATENT NO 9856454
SERIAL NO

14191675

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Abstract

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A method for rapid mincing of adipose tissues to isolate live cells in vitro is disclosed to overcome the drawbacks of a lead procedure of isolating live cells by holding a knife to mince adipose tissues, which can only isolate a small number of live cells and obtain low cell viability. The method includes: providing an adipose tissue; mincing the adipose tissue homogeneously by a mincing device; adding a reagent into the minced adipose tissue to perform hydrolysis; performing centrifuge and isolation; and removing a supernatant to obtain a cell pellet. Therefore, the time of mincing adipose tissues can be shortened, and contaminations caused by repeated use of the knife can be avoided. The method can be used for isolating live cells of adipose tissues to improve the number of live cells per unit weight of adipose tissues without reducing the cell viability.

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Patent Owner(s)

Patent OwnerAddress
BUDDHIST TZU CHI MEDICAL FOUNDATIONHUALIEN TAIWAN

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Chang, Yao-Jen Hualien County, TW 218 1492
Li, Dian-Kun Hualien County, TW 6 3
Pang, Cheng-Yoong Hualien County, TW 4 2
Sun, Li-Yi Hualien County, TW 3 3

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