MULTI-COMPARTMENT DEVICE FOR CELL CLONING AND METHOD OF PERFORMING THE SAME
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United States of America Patent
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Apr 29, 2015
filing date -
Aug 8, 2012
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Abstract
The problems associated with the traditional methods and devices in the field of cell cloning have been solved by the multi-compartment device and method in the present invention. The device combines the advantages of a traditional petri-dish and a traditional microplate. The multi-compartment device in the present invention comprises sidewalls, which are taller than openings of the multi-compartments. The cells in the suspension flow across the multi-compartments and seed inside the compartments during a plating process. The multi-compartment device in the present invention allows easier plating process, changing conditioned medium, and cell colony detachment and transfer. The multi-compartment device also minimizes the risk of cross-contamination during cloning process and during cell colony transfer. The invention also provides an exemplary method of using the multi-compartment device for cell cloning. In one aspect of the method, the multi-compartment device may be tilted before or after adding the cell suspension during plating process.
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