Method for quantifying different viral forms of the DNA of the HIV virus
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United States of America Patent
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Aug 20, 2015
app pub date -
Jun 12, 2013
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Jun 12, 2012
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Abstract
The invention relates to a method for quantifying linear forms of the DNA genome of the human immunodeficiency virus 1 (HIV-1) in a sample, wherein: a ligation reaction is performed with a double-stranded oligonucleotide suitable for ligation with the linear form of the HIV-1 DNA genome having a mature 3′ end and/or the linear form of the HIV-1 DNA genome having a non-mature 3′ end; a quantitative PCR is carried out from the ligation product obtained in the previous step; and the quantity of the linear form of the HIV-1 DNA genome having a mature 3′ end and/or the quantity of the linear form of the HIV-1 DNA genome having a non-mature 3′ end in the sample are deduced therefrom.
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