COMBINATION OF DSDNA BINDING DYE AND PROBES FOR CHARACTERIZATION OF SSDNA SEQUENCES

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United States of America Patent

APP PUB NO 20150218625A1
SERIAL NO

14428864

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Abstract

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This invention includes methods for analyzing single-stranded nucleic acid sequences, either RNA sequences or DNA sequences (ssDNA) utilizing dyes that fluoresce when associated with double strands, so-called DNA binding dyes or dsDNA-dyes. Methods according to this invention utilize a dsDNA-dye in combination with one or more hybridization probes that hybridize to a target nucleic acid sequence and that are labeled with a non-fluorescent quencher moiety, for example, a Black Hole quencher.

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Patent Owner(s)

Patent OwnerAddress
BRANDEIS UNIVERSITY415 SOUTH STREET WALTHAM MA 02454-9110 UNITED STATES OF AMERICA

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Jia, Yanwei Waltham, US 24 291
Rice, John E Quincy, US 18 70
Wangh, Lawrence J Auburndale, US 46 483

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